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Editorial Cuvillier

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Target‐oriented Bioprocess Optimization for Recombinant Protein Production in Bacillus megaterium

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Target‐oriented Bioprocess Optimization for Recombinant Protein Production in Bacillus megaterium (Volumen 68) (Tienda española)

Claudia Korneli (Autor)

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Lectura de prueba, PDF (460 KB)
Indice, PDF (90 KB)

ISBN-13 (Impresion) 9783954042890
ISBN-13 (E-Book) 9783736942899
Idioma Inglés
Numero de paginas 132
Laminacion de la cubierta mate
Edicion 1. Aufl.
Serie Schriftenreihe des Institutes für Bioverfahrenstechnik der Technischen Universität Braunschweig
Volumen 68
Lugar de publicacion Göttingen
Lugar de la disertacion Göttingen
Fecha de publicacion 10.12.2012
Clasificacion simple Tesis doctoral
Area Biología
Bioquímica, biología molecular, tecnología genética
Ingeniería mecánica y de proceso
Palabras claves Mikrobiologie und Biotechnologie, Allgemeine Verfahrenstechnik
Descripcion

The aim of this project was the development and targeted optimization of recombinant protein production with Bacillus megaterium. Based on a genetically defined model strain with plasmid based production of the gene for the green fluorescent protein (GFP), at first, the optimization of the basic culture parameters pH-value, temperature and dissolved oxygen concentration was performed. Further empirical analysis included an evaluation of different carbon sources and vitamin requirements. The use of GFP hereby enabled fast and simple detection of the production performance. Subsequently, recombinant protein production was also assessed on the cellular level – using system wide transcriptome and proteome analysis. Then, this was transferred to production processes at industrial scale, investigating limiting effects occurring in large scale bioreactors, i.e. heterogeneities in oxygen and substrate supply. Initially, a two compartment scale-down plant was developed for this purpose. Suboptimal feeding of the substrate induced extensive substrate- and oxygen gradients which had a negative effect on the GFP yield. With the help of advanced scale-down experiments, the metabolic origin of this detrimental production was investigated. In highly resolved time intervals the availability of free intracellular amino acids in B. megaterium was analyzed. Five strongly limiting amino acids were identified. In a subsequent large scale production the addition of these amino acids to the feed solution resulted in a 100 % increased GFP yield. Finally, the results from the model strain were successfully transferred to the production of a homologous protein, the levansucrase SacB. This enzyme is an important biocatalyst in the food industry for the production of prebiotic ingredients. In high cell density cultivations, a high product level, which was significantly higher than that of any other extracellular proteins, produced so far with B. megaterium, could be obtained. Hereby, the process allowed the use of raw glycerol, a waste product from biodiesel industry, as raw material for high titer and quality of the recombinant product.