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Induced mutagenesis and genetic engineering for the generation and selection of herbicide-resistant Alfalfa

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Induced mutagenesis and genetic engineering for the generation and selection of herbicide-resistant Alfalfa (Volumen 6) (Tienda española)

Pauline Moussard (Autor)

Previo

Lectura de prueba, PDF (130 KB)
Indice, PDF (83 KB)

ISBN-13 (Impresion) 9783954046027
ISBN-13 (E-Book) 9783736946026
Idioma Inglés
Numero de paginas 142
Laminacion de la cubierta Brillante
Edicion 1. Aufl.
Serie Schriftenreihe zur Physiologie und Biochemie der Pflanzen
Volumen 6
Lugar de publicacion Göttingen
Lugar de la disertacion Hohenheim
Fecha de publicacion 13.01.2014
Clasificacion simple Tesis doctoral
Area Biología
Bioquímica, biología molecular, tecnología genética
Agricultura
Palabras claves alfalfa, Medicago sativa, weeds, herbicide, herbicide-resistance, mutagenesis, selection, ethylmethane sulfonate, acetolactate synthase, hydroxyphenylpyruvate-dioxygenase, glutamine synthase, in vitro tissue culture, somatic embryogenesis, genetic transformation, Agrobacterium tumefaciens, Allgemeine Biologie, Pflanzenproduktion
Descripcion

Weeds in alfalfa (Medicago sativa) fields reduce both the yield and the quality of the culture and therefore the value of the crop. Nowadays there is no suitable conventional solution for the efficient control of weeds in alfalfa. Increasing concerns about weed shifts and the recent appearance of glyphosate-resistant weeds in alfalfa may require alfalfa lines resistant to other broad-spectrum herbicides, with different sites of actions. Therefore, the aim of the present work was the development of herbicide-resistant alfalfa lines to allow the application of broad-spectrum herbicides on growing alfalfa. Towards this goal a first approach was chosen based on the selection of natural or induced (ethylmethane sulfonate mutagenesis) plant mutants with resistance to acetolactate synthase (ALS) inhibitors. On the other hand, a transgenic approach using three different, herbicide resistance-promoting genes (hydroxyphenylpyruvate-dioxygenase, glutamine synthase, and ALS) was developed and herbicide-resistant alfalfa lines were successfully produced. For both approaches, a robust and efficient in vitro tissue culture was optimized.