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Editorial Cuvillier

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Conformational changes in formylglycine-generating enzyme during the catalytic cycle: Role of reducing agent and calcium

Impresion
EUR 33,50

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EUR 23,45

Conformational changes in formylglycine-generating enzyme during the catalytic cycle: Role of reducing agent and calcium (Tienda española)

Md Sarfaraz Alam (Autor)

Previo

Lectura de prueba, PDF (230 KB)

ISBN-13 (Impresion) 9783736991101
ISBN-13 (E-Book) 9783736981102
Idioma Inglés
Numero de paginas 158
Laminacion de la cubierta Brillante
Edicion 1. Aufl.
Lugar de publicacion Göttingen
Lugar de la disertacion Göttingen
Fecha de publicacion 21.09.2015
Clasificacion simple Tesis doctoral
Area Bioquímica, biología molecular, tecnología genética
Palabras claves Formylglycine generation enzyme, Multiple sulfatase deficiency, in vitro activity
Descripcion

Formylglycine generation, a unique process necessary for the activation of sulfatases, is a cotranslational event conserved from pro- to eukaryotes and is defined by the modification of the cysteine residue in the CxPxR motif of largely unfolded sulfatase polypeptides to Cα-formylglycine (FGly). This process is confined to the endoplasmic reticulum (ER) in eukaryotes and catalyzed by the formylglycine-generating enzyme (FGE), a resident protein of the ER. Mutations in FGE, that impair FGly generation, lead to production of inactive sulfatases that manifests into an inherited metabolic disorder termed multiple sulfatase deficiency (MSD) in humans. Although FGE has been proposed to function as a cofactor independent monooxygenase, the precise mechanism is not yet clear. In the present study, a baculovirus-based method for expression and purification of recombinant FGE from cultured insect cells was established and the recombinant FGE was characterized in more detail.