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A Multi-omics Perspective on Osmoadaptation and Osmoprotection in Bacillus subtilis

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A Multi-omics Perspective on Osmoadaptation and Osmoprotection in Bacillus subtilis (Band 1)

Michael Kohlstedt (Autor)

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Inhaltsverzeichnis, PDF (71 KB)
Leseprobe, PDF (340 KB)

ISBN-13 (Printausgabe) 9783954047987
ISBN-13 (E-Book) 9783736947986
Sprache Englisch
Seitenanzahl 136
Umschlagkaschierung matt
Auflage 1. Aufl.
Buchreihe Hochschulschriften - Institut für Systembiotechnologie, Universität des Saarlandes
Band 1
Erscheinungsort Göttingen
Promotionsort Saarbrücken
Erscheinungsdatum 12.09.2014
Allgemeine Einordnung Dissertation
Fachbereiche Biologie
Mikrobiologie und Biotechnologie
Schlagwörter Bacillus subtilis, Osmoregulation, Kompatible Solute, Systembiologie, Multiomics, multi-omics systems biology, osmotic stress, compatible solutes, Biotechnologie
Beschreibung

In its natural environment, the soil bacterium Bacillus subtilis permanently encounters nutrient limitations and increases in osmotic stress. The present study investigated the systemswide response of B. subtilis to different simultaneously imposed stresses by combining
chemostat experiments under conditions of carbon and nitrogen limitation, ionic and nonionic osmotic stress and osmoprotection with multi-omics analyses of the transcriptome, proteome, metabolome and fluxome. Surprisingly, the flux through central carbon and energy metabolism is very robust under all conditions studied. The key to achieve this robustness is the increased production of several enzymes of central carbon metabolism to compensate for their reduced activity in the presence of high salt. A major response of the cell during osmotic stress is the production of the compatible solute proline through the concerted adjustment of multiple reactions around the 2-oxoglutarate node, which drives metabolism towards the precursor glutamate. Co-regulations between the individual cellular components under the investigated stress conditions indicate that the fine-tuning of the transcriptional and metabolic networks involves functional modules that outreach single pathways. Additionally, the work shortly describes the salinity-decoupled fed-batch production of the compatible solute ectoine in Corynebacterium glutamicum and the contribution of precursor amino acids of the aspartate family.