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Whole plant in vivo and in silico metabolic flux analysis: towards biotechnological application

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Whole plant in vivo and in silico metabolic flux analysis: towards biotechnological application (Volumen 3)

Veronique Starck (Autor)

Previo

Lectura de prueba, PDF (1,3 MB)
Indice, PDF (550 KB)

ISBN-13 (Impresion) 9783736999480
ISBN-13 (E-Book) 9783736989481
Idioma Inglés
Numero de paginas 242
Laminacion de la cubierta mate
Edicion 1.
Serie Hochschulschriften - Institut für Systembiotechnologie, Universität des Saarlandes
Volumen 3
Lugar de publicacion Göttingen
Lugar de la disertacion Saarbrücken
Fecha de publicacion 07.02.2019
Clasificacion simple Tesis doctoral
Area Biología
Microbiología y biotécnica
Palabras claves Arabidopsis thaliana, biotechnology, Biotechnologie, transgenic, transgen, metabolic modelling, metabolische Modellierung, metabolic network, metabolisches Netzwerk, metabolism, Metabolismus, elementary flux modes, elementary modes, Elementarmodi, omics-data, Omics-Daten, plant growth, Pflanzenwachstum, isotopes, Isotope, isotopic labelling, Isotopenlabeling, seedling, Sämling, reactor, Reaktor, INST-MFA, INST-MFA, flux, Fluss, genome-scale, genomweit, stoichiometric network, stöchiometrisches Netzwerk, diurnal, tagaktiv, physiology, Physiologie, photoautotroph, Fotoautotroph, metabolic engineering, metabolisches Engineering, redox, Redox, biomass synthesis, Biomasse-Synthese, leaves, Blätter, isotopomer, isotopomer, parameter continuation, Parameterfortsetzung, flux analysis, Flussanalyse, plants, Pflanzen, plant physiology, Pflanzenphysiologie, crop, Gewächs, systems biology, Systembiologie, Oryza sativa, Oryza sativa, non-stationary, instationär, 13C, 13C, metabolic flux analysis, Stoffwechselflussanalyse, imazapyr, imazapyr, herbicide, Herbizid, futile cycling, Substratzyklus, green biotechnology, grüne Biotechnologie, rice, Reis, thale cress, Acker-Schmalwand, arabidopsis, Arabidopsis, cress, Kresse
Descripcion

In the present work, both in silico and in vivo methods for flux analysis in plants were successfully developed and applied for enhanced understanding of plant physiology. Taken together, the in silico metabolic simulations provide detailed molecular insights into plant functioning, particularly by linking in vivo with in silico data. The knowledge gained from such a systems-biological approach, together with the proposed high potential of plants as biotechnological production platforms, especially for compounds requiring much redox power, will help to establish plants as biotechnological factories.
For the first time, the in vivo metabolism of an agriculturally relevant crop, O. sativa, was investigated, through non-stationary 13C-metabolic flux analysis. This allowed elucidation of the in vivo intracellular carbon partitioning in rice plants and of the plants’ necessity for futile cycling of resources, thus, contributing significantly to our current knowledge on plant metabolism. In addition, the effect of imazapyr, an industrially relevant herbicide, on rice metabolism was inspected using the newly established workflow. This first real-life case-study provides a valuable proof-of-principle and enabled a deeper understanding of the immediate metabolic effects of the treatment. This method can now be adopted to other crops, cell lines and stress inducers, such as abiotic stresses, herbicides and fungicides, and therefore, has great potential in green biotechnology.