Cookies help us deliver our services. By using our services, you agree to our use of cookies.
De En Es
Kundenservice: +49 (0) 551 - 547 24 0

Cuvillier Verlag

Publications, Dissertations, Habilitations & Brochures.
International Specialist Publishing House for Science and Economy

Cuvillier Verlag

Premiumpartner
De En Es
Titelbild-leitlinien
Whole plant in vivo and in silico metabolic flux analysis: towards biotechnological application

Hard Copy
EUR 67.90

E-book
EUR 47.50

Whole plant in vivo and in silico metabolic flux analysis: towards biotechnological application (Volume 3)

Veronique Starck (Author)

Preview

Extract, PDF (1.3 MB)
Table of Contents, PDF (550 KB)

ISBN-13 (Hard Copy) 9783736999480
ISBN-13 (eBook) 9783736989481
Language English
Page Number 242
Lamination of Cover matt
Edition 1.
Book Series Hochschulschriften - Institut für Systembiotechnologie, Universität des Saarlandes
Volume 3
Publication Place Göttingen
Place of Dissertation Saarbrücken
Publication Date 2019-02-07
General Categorization Dissertation
Departments Biology
Microbiology and biotechnology
Keywords Arabidopsis thaliana, biotechnology, Biotechnologie, transgenic, transgen, metabolic modelling, metabolische Modellierung, metabolic network, metabolisches Netzwerk, metabolism, Metabolismus, elementary flux modes, elementary modes, Elementarmodi, omics-data, Omics-Daten, plant growth, Pflanzenwachstum, isotopes, Isotope, isotopic labelling, Isotopenlabeling, seedling, Sämling, reactor, Reaktor, INST-MFA, INST-MFA, flux, Fluss, genome-scale, genomweit, stoichiometric network, stöchiometrisches Netzwerk, diurnal, tagaktiv, physiology, Physiologie, photoautotroph, Fotoautotroph, metabolic engineering, metabolisches Engineering, redox, Redox, biomass synthesis, Biomasse-Synthese, leaves, Blätter, isotopomer, isotopomer, parameter continuation, Parameterfortsetzung, flux analysis, Flussanalyse, plants, Pflanzen, plant physiology, Pflanzenphysiologie, crop, Gewächs, systems biology, Systembiologie, Oryza sativa, Oryza sativa, non-stationary, instationär, 13C, 13C, metabolic flux analysis, Stoffwechselflussanalyse, imazapyr, imazapyr, herbicide, Herbizid, futile cycling, Substratzyklus, green biotechnology, grüne Biotechnologie, rice, Reis, thale cress, Acker-Schmalwand, arabidopsis, Arabidopsis, cress, Kresse
Description

In the present work, both in silico and in vivo methods for flux analysis in plants were successfully developed and applied for enhanced understanding of plant physiology. Taken together, the in silico metabolic simulations provide detailed molecular insights into plant functioning, particularly by linking in vivo with in silico data. The knowledge gained from such a systems-biological approach, together with the proposed high potential of plants as biotechnological production platforms, especially for compounds requiring much redox power, will help to establish plants as biotechnological factories.
For the first time, the in vivo metabolism of an agriculturally relevant crop, O. sativa, was investigated, through non-stationary 13C-metabolic flux analysis. This allowed elucidation of the in vivo intracellular carbon partitioning in rice plants and of the plants’ necessity for futile cycling of resources, thus, contributing significantly to our current knowledge on plant metabolism. In addition, the effect of imazapyr, an industrially relevant herbicide, on rice metabolism was inspected using the newly established workflow. This first real-life case-study provides a valuable proof-of-principle and enabled a deeper understanding of the immediate metabolic effects of the treatment. This method can now be adopted to other crops, cell lines and stress inducers, such as abiotic stresses, herbicides and fungicides, and therefore, has great potential in green biotechnology.