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Editorial Cuvillier

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Editorial Cuvillier

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Advanced Diffusion Studies of Active Enzymes and Nanosystems

Impresion
EUR 52,90

E-Book
EUR 37,90

Advanced Diffusion Studies of Active Enzymes and Nanosystems (Tienda española)

Jan-Philipp Günther (Autor)

Previo

Lectura de prueba, PDF (120 KB)
Indice, PDF (85 KB)

ISBN-13 (Impresion) 9783736973640
ISBN-13 (E-Book) 9783736963641
Idioma Inglés
Numero de paginas 190
Laminacion de la cubierta Brillante
Edicion 1
Lugar de publicacion Göttingen
Lugar de la disertacion Stuttgart
Fecha de publicacion 03.02.2021
Clasificacion simple Tesis doctoral
Area Física
Física atómica y molecular, física plasma y de gases
Química
Físicoquimica
Biofísica
Palabras claves Enzyme, Alkalische Phosphatase, Aldolase, Urease, ATP Synthase, F1-ATPase, Viren, M13 Bakteriophagen, Physikalische Chemie, Biophysikalische Chemie, Aktive Materie, Aktive Diffusion, Erhöhte Diffusion, Selbstphorese, Selbstdiffusiophorese, Diffusionsmessungen, Fluoreszenzkorrelationsspektroskopie, FCS, FCCS, Fluoreszenzlöschung, Kernspinresonanzspektroskopie, NMR, PFG-NMR, Spin-Relaxation, Partikelverfolgung, Konvektion, Regime kleiner Reynolds-Zahlen, Schwimmen, Selbstantrieb, Homogene Katalyse, Click Chemie, Molekulare Katalysatoren, Artefakt, Fehlinterpretation, Enzym-Phagen-Kolloid, Enzymatische Mikropumpe, Nanosysteme, Biohybridsysteme, Biokonjugation, Selbstassemblierung, Enzymes, alkaline phosphatase, aldolase, urease, ATP synthase, F1-ATPase, virus, M13 bacteriophage, physical chemistry, biophysical chemistry, active matter, active diffusion, enhanced diffusion, self-phoresis, self-diffusiophoresis, diffusion measurements, fluorescence correlation spectroscopy, FCS, FCCS, fluorescence quenching, nuclear magnetic resonance spectroscopy, NMR, PFG-NMR, spin relaxation, particle tracking, convection, low Reynolds number regime, swimming, self-propulsion, homogenous catalysis, click chemistry, molecular catalysts, artefact, misinterpretation, enzyme-phage-colloid, enzymatic micropump, nanosystem, biohybrid system, bioconjugation, self-assembly, Brownsche Bewegung, Nanoskala, Feldgradienten, Pulse Sequences, Pulssequenzen
URL para pagina web externa https://pf.is.mpg.de/
Descripcion

Enzymes are fascinating chemical nanomachines that catalyze many reactions, which are essential for life. Studying enzymes is therefore important in a biological and medical context, but the catalytic potential of enzymes also finds use in organic synthesis. This thesis is concerned with the fundamental question whether the catalytic reaction of an enzyme or molecular catalyst can cause it to show enhanced diffusion. Diffusion measurements were performed with advanced fluorescence correlation spectroscopy (FCS) and diffusion nuclear magnetic resonance (NMR) spectroscopy techniques. The measurement results lead to the unraveling of artefacts in enzyme FCS and molecular NMR measurements, and thus seriously question several recent publications, which claim that enzymes and molecular catalysts are active matter and experience enhanced diffusion. In addition to these fundamental questions, this thesis also examines the use of enzymes as biocatalysts. A novel nanoconstruct – the enzyme-phage-colloid (E-P-C) – is presented, which utilizes filamentous viruses as immobilization templates for enzymes. E-P-Cs can be used for biocatalysis with convenient magnetic recovery of enzymes and serve as enzymatic micropumps. The latter can autonomously pump blood at physiological urea concentrations.