|Zahn-, Mund- und Kieferheilkunde||9|
|Biochemie, Molekularbiologie, Gentechnologie||107|
|Ernährungs- und Haushaltswissenschaften||40|
|Land- und Agrarwissenschaften||966|
|Umweltforschung, Ökologie und Landespflege||131|
5. Auflage bestellen
Land- und Agrarwissenschaften
A survey was conducted to determine the occurrence and distribution of plant-parasitic
nematodes and associated endophytic fungi and bacterial antagonists on coffee in
Ethiopia. Soil and root samples were collected from 15 coffee growing agroecologies
during the wet season in August 2004 and dry season in April 2006. The dominant
plant-parasitic nematode genera found were Helicotylenchus, Scutellonema,
Rotylenchus, Xiphinema, Heterodera, Tylenchorhynchus and Quinisulcius.
Helicotylenchus predominated throughout the area (65-74%), followed by Xiphinema
(29-40%). Nematode densities were generally higher in the wet season than in the dry
season, however, relative abundance of nematode taxa was in the same order. For the
major plant-parasitic nematode genera, specimens were identified to the following
species: Helicotylenchus dihystera, H. multicinctus, H. californicus, H. gerti, Xiphinema
insigne, X. basilgoodeyi, Scutellonema paralabiatum, Rotylenchus unisexus,
Tylenchorhynchus agri and Quinisulcius capitatus. Nematodes considered to be of
economic importance and involved in coffee decline were not detected in this survey.
A total of 128 fungi growing endophytically were obtained from surface sterilized coffee
roots. The most frequently detected genera were Fusarium and Trichoderma.
Nematicidal effects of culture filtrates of these isolates on M. incognita juveniles were
observed after 24 h of incubation. Juvenile inactivity ranged from 28% to 81% in which
Trichoderma strain ET2 showed the highest level of inactivity up to 81%. Same isolates
were tested for their pathogenicity towards M. incognita eggs. Isolates EF1, EF3, EF5,
EF6, EF8, EF10, EF11, EU2 and EU4 showed the highest ability to infect eggs with
values up to 89%. The percentage of egg pathogenicity was highest for Fusarium spp.
Results of replicated greenhouse experiments demonstrated that isolates of Fusarium
and Trichoderma reduced the number root-knot nematode egg masses up to 50%. Of
all the tested isolates, the F. oxysporum isolate EF11 significantly reduced the number
of egg masses in all sets of experiments. Reduction in nematode penetration ranging up
to 30% was also recorded in the present study for isolates EF11 and EF10.
Endophytic bacteria were isolated and identified by Fatty Acid Methyl Ester-Gas
Chromatography (FAME-GC). A total of 201 and 114 endophytic bacteria were isolated
and identified during the wet and dry seasons, respectively. The most abundant genera
found were Pseudomonas, Bacillus, Agrobacterium, Stenotrophomonas and
Enterobacter. Higher population size densities ranging from 5.2 × 103 to 2.07 × 106 cfu g-
1 fresh root were observed during the wet season. Culture filtrates of the bacterial
isolates showed nematicidal effects ranged between 38% and 98%. The active strains
were: Agrobacterium radiobacter, Bacillus pumillus, B. brevis, B. megaterium, B.
mycoides, B. licheniformis, Chryseobacterium balustinum, Cedecea davisae,
Cytophaga johnsonae, Lactobacillus paracasei, Micrococcus luteus, M. halobius,
Pseudomonas syringae and Stenotrophomonas maltophilia. Bacillus pumillus and B.
mycoides were the most effective strains in reducing the number of egg masses and
galls up to 39 and 33%, respectively.