Cuvillier Verlag

35 Jahre Kompetenz im wissenschaftlichen Publizieren
Internationaler Fachverlag für Wissenschaft und Wirtschaft

Cuvillier Verlag

De En Es
New Algorithms for Single Particle Cryo Electron Microscopic Image Processing

Printausgabe
EUR 36,80

New Algorithms for Single Particle Cryo Electron Microscopic Image Processing

Jan-Martin Kirves (Autor)

Vorschau

Inhaltsverzeichnis, PDF (31 KB)
Leseprobe, PDF (220 KB)

ISBN-13 (Printausgabe) 9783954049073
Sprache Englisch
Seitenanzahl 174
Umschlagkaschierung matt
Auflage 1. Aufl.
Erscheinungsort Göttingen
Promotionsort Göttingen
Erscheinungsdatum 06.01.2015
Allgemeine Einordnung Dissertation
Fachbereiche Informatik
Biologie
Mikrobiologie und Biotechnologie
Schlagwörter cyro electron microscopy, validation, quality criteris, sample optimization, software
Beschreibung

In this thesis four approaches to increase the quality and assure the validity of three-dimensional (3D) models generated within the single particle cryo-electron microscopy (CryoEM) workflow are introduced. ProteoPlex is a high-throughput technique and software tool for the evaluation of the most stabilizing biochemical conditions of macromolecular complexes. The validation technique 3D correlation of tilted pairs (3DCTP) can be applied alongside the single particle refinement cycle to detect model bias utilizing tilted pair images. A new analysis method identifies the presence of overfitting in high resolution CryoEM 3D structures. Finally, a set of criteria covering different model quality aspects of the generated 3D structure is introduced.