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New Algorithms for Single Particle Cryo Electron Microscopic Image Processing

Hard Copy
EUR 36.80

New Algorithms for Single Particle Cryo Electron Microscopic Image Processing (English shop)

Jan-Martin Kirves (Author)

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ISBN-13 (Hard Copy) 9783954049073
Language English
Page Number 174
Lamination of Cover matt
Edition 1. Aufl.
Publication Place Göttingen
Place of Dissertation Göttingen
Publication Date 2015-01-06
General Categorization Dissertation
Departments Informatics
Biology
Microbiology and biotechnology
Keywords cyro electron microscopy, validation, quality criteris, sample optimization, software
Description

In this thesis four approaches to increase the quality and assure the validity of three-dimensional (3D) models generated within the single particle cryo-electron microscopy (CryoEM) workflow are introduced. ProteoPlex is a high-throughput technique and software tool for the evaluation of the most stabilizing biochemical conditions of macromolecular complexes. The validation technique 3D correlation of tilted pairs (3DCTP) can be applied alongside the single particle refinement cycle to detect model bias utilizing tilted pair images. A new analysis method identifies the presence of overfitting in high resolution CryoEM 3D structures. Finally, a set of criteria covering different model quality aspects of the generated 3D structure is introduced.