Molekularphysiologische Untersuchungen zellulärer biochemischer Prozesse (English shop)

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This habilitation thesis investigates biochemical processes in living cells using advanced fluorescence microscopy techniques. The research focuses on quantitative analysis of cellular signal transduction and the development and application of optical biosensors. Particular emphasis is placed on Förster resonance energy transfer (FRET) and fluorescence lifetime imaging microscopy (FLIM), which enable spatially and temporally resolved measurements of molecular interactions. Investigated processes include epidermal growth factor receptor activation, peroxisomal fatty acid oxidation, and cytoskeletal regulation. Further research addresses neuronal differentiation, neurite outgrowth, and the molecular mechanisms underlying neurodegenerative diseases. Special attention is given to tau protein aggregation and cellular protein quality control mechanisms, including chaperone activity and the ubiquitin-proteasome system. The thesis also presents technological improvements in fluorescence-based measurement methods and demonstrates their importance for understanding cellular functions and biochemical interactions.