Pregnancy-associated glycoprotein (PAG) im Blut vom Schaf und Östrogen-Konzentration im Kot der Ziege als Hilfsmittel zur Trächtigkeitsdiagnose (English shop)

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This dissertation investigates the suitability of hormonal and placental biomarkers for pregnancy diagnosis in sheep and goats. The research focuses on pregnancy-associated glycoprotein (PAG) concentrations in sheep blood plasma and total estrogen concentrations in goat feces. Using radioimmunoassay, PAG concentrations are analyzed in 66 pregnant ewes of different breeds throughout gestation and after parturition. The findings demonstrate that reliable differentiation between pregnant and non-pregnant animals is possible from the fourth week of gestation. Furthermore, the influence of litter size, sheep breed, lamb sex, and birth weight on PAG concentrations is examined. A second research focus involves determining total estrogen concentrations in blood, urine, and feces of pregnant goats using enzyme immunoassay. Particular attention is given to the effects of sample storage duration and temperature on fecal hormone concentrations. The results confirm the potential of both biomarkers for pregnancy diagnosis and highlight the importance of noninvasive hormone assessment in reproductive monitoring of small ruminants.