Untersuchung der Einzelmoleküldynamik mit multidimensionaler Fluoreszenzspektroskopie (English shop)

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The investigation of individual molecules provides new opportunities to understand dynamic processes and structural properties of biological systems. This dissertation focuses on multidimensional fluorescence spectroscopy, particularly Multiparameter Fluorescence Detection (MFD), which enables the simultaneous measurement of fluorescence intensity, fluorescence lifetime, and anisotropy at the single-molecule level.

Using fluorescently labeled oligonucleotides, Sylvia Berger investigates interactions between fluorescent dyes and nucleic acids, as well as Fluorescence Resonance Energy Transfer (FRET). By combining different fluorescence parameters, the study enables precise measurements of molecular distances and the identification of distinct molecular subpopulations within heterogeneous systems.

Another major focus is the investigation of HIV-1 reverse transcriptase and its interactions with nucleic acid substrates. The research identifies three structurally distinct enzyme-substrate complexes, providing new insights into their molecular structures and dynamics. The findings demonstrate the potential of single-molecule fluorescence spectroscopy for structural characterization and the investigation of biological macromolecules under physiological conditions.