Optische Messung der Fließgeschwindigkeit von Erythrozyten zur Erfassung der Mikrozirkulation (English shop)

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The investigation of microcirculation can make a decisive contribution to the early diagnosis of serious diseases and life-threatening conditions as well as to the health-promoting treatment of patients. Measuring blood flow via the velocity of the particles present in the blood in the smallest blood vessels represents one way of precisely recording microcirculation locally. At the nail fold of the fingers, the movement of erythrocytes in the capillaries lying just beneath the skin surface can be made visible for this purpose.

In this work, following a comparison of established measurement techniques (image processing, laser Doppler anemometry, PIV), a method based on the spatial filtering principle is presented. With this method, the velocity of red blood cells can be measured with high temporal and spatial resolution and continuously in time, even in curvilinear capillary structures. The measurement system set up for this purpose consists of an objective lens, a CMOS camera and a measurement computer.

To eliminate disturbing finger movements during the measurement, a highly efficient motion correction is employed, so that, together with the velocity measurement, real-time measurement of the erythrocyte flow velocity is possible. The continuously recorded information is thus reduced to the pure velocity time course and, despite the high temporal resolution, also permits very long-lasting measurements, so that the essential velocity components (pulse, vasomotion, trend) of microcirculation can be captured.