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Metabolic Network Analysis of the Cell Factory Aspergillus niger

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Metabolic Network Analysis of the Cell Factory Aspergillus niger (Volume 47)

Guido Melzer (Author)

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ISBN-13 (Printausgabe) 3869554568
ISBN-13 (Hard Copy) 9783869554563
ISBN-13 (eBook) 9783736934566
Language English
Page Number 180
Edition 1 Aufl.
Book Series Schriftenreihe des Institutes für Bioverfahrenstechnik der Technischen Universität Braunschweig
Volume 47
Publication Place Göttingen
Place of Dissertation TU Braunschweig
Publication Date 2010-09-10
General Categorization Dissertation
Departments Biology
Keywords Aspergillus niger, Flux Design, in silico, Metabolic network analysis, Elementary flux modes, gene amplification targets, gene attenuation targets, flux correlation, optimal yield, optimal fluxes, cell factories, Corynebacterium glutamicum, lysine production, sucrase, fructofuranosidase, epoxide hydrolase
Description

In the present work, a systems biology approach is presented, which enables the prediction of potential genetic targets and optimal pathways for protein production within a metabolic network, and thus, makes an important contribution to the rational strain optimization of micro-organisms. For this, elementary flux modes analysis was carried out using a metabolic model of Aspergillus niger, which was condensed from the genome based metabolic model. Hereby, a new approach was developed for the design of cell factories by the analysis of metabolic flux correlations between metabolic enzymes. This allowed the in silico prediction of deletion and amplification targets and thus provides an important prerequisite for rational strain optimization.
The network analysis was carried out using various target products under varying nutrient conditions. The approach revealed that the success of identification of genetic targets depends on the differentiation of biological states, the growth-associated or non-growth associated production of the target proteins. Only a few targets, such as the pathways of protein glycosylation and protein biosynthesis can be identified as independent of the biological state. The growth-associated targets include inter alia the pentose-phosphate pathway (amplification target) and the reactions of the tricarboxylic acid cycle (attenuation target). The results of this systems biology approach could be validated by enzyme kinetic studies and analyses of intracellular metabolic fluxes using metabolite balancing and continuous cultivations. In addition, metabolic networks of several industrially relevant hosts were investigated using this in silico approach and essential differences were elaborated. Comparisons with experimental studies for rational strain optimization of Corynebacterium glutamicum for lysine production support the applicability of this novel in silico approach.