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Cuvillier Verlag

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Extending the scope of protein synthesis by a novel auxiliary‐based Native Chemical Ligation strategy

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Extending the scope of protein synthesis by a novel auxiliary‐based Native Chemical Ligation strategy (English shop)

Christina Nadler (Author)

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ISBN-13 (Hard Copy) 9783954045044
ISBN-13 (eBook) 9783736945043
Language English
Page Number 198
Lamination of Cover matt
Edition 1. Aufl.
Publication Place Göttingen
Place of Dissertation Göttingen
Publication Date 2013-09-18
General Categorization Dissertation
Departments Chemistry
Organic chemistry
Keywords Organische Chemie, Peptidchemie, Festphasensynthese, SPPS, Native Chemical Ligation, cystein-freie Native Chemical Ligation, Ligationsauxiliar, Photolabilität, artifizielle Aminosäuren, Histidinmimetika, Single Amino Acid Chelate, Kupfer-katalysierte Azid-Alkin-Cycloaddition, Fluoreszenzspektroskopie, Zinksensoren, Zinkfingerpeptide, Zif268, Substratanalogon, Lipoxygenase.
Description

There is a constant need for developing improved methods for introducing artificial functionalities into peptides and proteins, as the modification of peptides and proteins is one of the major routes to investigate biological function in vitro and in vivo, e.g. by introduction of spin labels or fluorophores. To improve the synthetic accessibility of chemically modified peptides and proteins a new cysteine-free Native Chemical Ligation strategy based on a photocleavable auxiliary was developed and successfully implemented. In addition, a novel protocol for labeling peptides and proteins by introducing artificial, histidine-mimicking amino acids was devised. These triazole-based building blocks were utilized for the introduction of additional metal binding sites into peptides as well as for the development of peptidic zinc sensors based on zinc finger peptide Zif268.