Cookies help us deliver our services. By using our services, you agree to our use of cookies.
De En Es
Kundenservice: +49 (0) 551 - 547 24 0

Cuvillier Verlag

Publications, Dissertations, Habilitations & Brochures.
International Specialist Publishing House for Science and Economy

Cuvillier Verlag

Premiumpartner
De En Es
Titelbild-leitlinien
In situ-downstream processing of recombinant histidine-tagged proteins  from cultivations of Bacillus megaterium

Hard Copy
EUR 36.50

E-book
EUR 25.60

In situ-downstream processing of recombinant histidine-tagged proteins from cultivations of Bacillus megaterium (Volume 79)

Johannes Gädke (Author)

Preview

Table of Contents, PDF (52 KB)
Extract, PDF (73 KB)

ISBN-13 (Hard Copy) 9783736995512
ISBN-13 (eBook) 9783736985513
Language English
Page Number 128
Edition 1.
Book Series Schriftenreihe des Institutes für Bioverfahrenstechnik der Technischen Universität Braunschweig
Volume 79
Publication Place Göttingen
Place of Dissertation Braunschweig
Publication Date 2017-06-15
General Categorization Dissertation
Departments Microbiology and biotechnology
Keywords In situ, downstream processing, purification, SPION, superparamagnetism, Bacillus megaterium, scale-up
Description

Purification of (recombinant) proteins for industrial and pharmaceutical use is accompanied by high costs and difficulties in the scale-up with the necessity of many purification steps. This thesis demonstrates the use of functionalized superparamagnetic iron oxide nanoparticles for the purification of recombinant histidine-tagged proteins directly from a growing culture of the gram-positive bacterium Bacillus megaterium. The separation was performed using commercial hand held magnets. Regenerability and reusability are shown in shake flask scale. Automation of the process is demonstrated at lab scale bioreactors using two model proteins, Protein A and the antibody fragment α-Lysozyme D1.3scFv. The process demonstrates a quick and easy way to yield a product of high purity within a short period of time.