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Application of reverse genetic systems to study Beet soil-borne mosaic virus and Beet necrotic yellow vein virus molecular biology, the interaction of species and their use as biotechnological tool

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Application of reverse genetic systems to study Beet soil-borne mosaic virus and Beet necrotic yellow vein virus molecular biology, the interaction of species and their use as biotechnological tool (Volume 54)

Marlene Laufer (Author)

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ISBN-13 (Hard Copy) 9783736998148
ISBN-13 (eBook) 9783736988149
Language English
Page Number 154
Edition 1.
Book Series Aus dem Institut für Zuckerrübenforschung Göttingen
Volume 54
Publication Place Göttingen
Place of Dissertation Göttingen
Publication Date 2018-06-18
General Categorization Dissertation
Departments Agricultural science
Keywords Beet soil-borne mosaic virus, Beet necrotic yellow vein virus, Benyviridae, RNA viruses, interaction of species, sugar beet, rhizomania, plant resistance, colonisation strategy, reassortants, recombinants, mixed infection, co infection exclusion, super infection exclusion, reverse genetic, infectious cDNA full length clones, agroinoculation, viral vectors, fluorescence labelling, fluorescent proteins, confocal laser scanning microscopy, virus induced gene silencing
Description

Two members of the Benyviridae family, Beet soil borne mosaic virus (BSBMV) and Beet necrotic yellow vein virus (BNYVV), possess identical genome organisation, host range and high sequence similarity. To understand functional differences in molecular biology, pathogenicity mechanisms, symptom expression as well as interaction with the host and between viral species, a reverse genetic system represents a prerequisite. Infectious cDNA full length clones of both viruses were constructed by isothermal in vitro recombination. Artificial formation of BNYVV and BSBMV RNA1+2 reassortants were viable and spread long distance in N. benthamiana. Small genomic RNAs were exchangeable and systemically infected B. macrocarpa. Moreover, fluorescence labelled full length clones were achieved by replacing a part of the RNA2 encoded coat protein read through domain. Co infection experiments with labelled BSBMV and BNYVV showed that both viruses remained spatially separated after N. benthamiana agroinoculation. In contrast, a mixture of BSBMV with an unrelated virus resulted in no co infection exclusion. In super infection experiments, BSBMV and BNYVV were unable to establish a secondary infection in plants that were previously infected with BNYVV or BSBMV. In addition, a virus induced gene silencing (VIGS) system was developed. Both viruses were equipped with fragments of the magnesium chelatase subunit H and phytoene desaturase genes. Silencing phenotypes in N. benthamiana induced by both target genes, were comparable to those described in the literature.