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Shining bright: Fluorescent tools for drug discovery, development and delivery

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EUR 58.40

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Shining bright: Fluorescent tools for drug discovery, development and delivery

Bettina Olshausen (Author)

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ISBN-13 (Hard Copy) 9783736998162
ISBN-13 (eBook) 9783736988163
Language English
Page Number 216
Lamination of Cover glossy
Edition 1.
Publication Place Göttingen
Place of Dissertation Karlsruhe
Publication Date 2018-06-25
General Categorization Dissertation
Departments Organic chemistry
Biochemistry, molecular biology, gene technology
Keywords fluorescence, dye, fluorescent dyes, drug development, drug discovery, drug delivery, BODIPY, fluorescence microscopy, imaging, chemical biology, oligonucleotides, DNA/RNA traffic lights, siRNA, visualization, drug uptake, Lipinski, rule of 5, melanoma, early detection, melanoma diagnostics, cyanin dye, styryl dye, fluorescein, specific uptake, biologically active molecules, targeted drug delivery, polymer, nanocarrier, EPF effect, tumor, tumor targeting, side effects, drug efficacy, disease, drug target, synthesis
Description

In chemical biology, the fluorescent labeling of molecules and usage of fluorescence-based imaging techniques for visualizing molecular interactions at a subcellular level is a key feature and contributes significantly to the elucidation of disease mechanisms. Furthermore, potential drug target sites can be identified, drugs acting on such sites can be developed, and by their labeling, the interactions of drugs and targets can be uncovered. In this work, innovative approaches for the fluorescent labeling of proteins, nucleic acids, and drugs are presented. A novel methodology for the facile synthesis of a versatile, functionalized, water-soluble, and tunable fluorescent probe has been developed. As a class of promising drug candidates, oligonucleotides were labeled in a dual-color readout DNA/RNA “Traffic Lights” concept that allows reliable tracking of cellular delivery, target binding, and structural integrity of nucleic acids. To ensure efficient early detection of diseases, a melanocyte-specific compound has been synthesized with the goal of enabling melanoma detection via non-invasive fluorescence microscopy. Once a cancerous disease with a solid tumor is detected, the efficient transport of a drug to the tumor site and local accumulation is of meaningful impact. A polymeric nanocarrier system for targeted tumor transport and triggered drug release has been developed.