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Cellular fluctuations, alpha-fetoprotein expression, and the question of stem cells  in experimental liver regeneration and hepatocarcinogenesis

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Cellular fluctuations, alpha-fetoprotein expression, and the question of stem cells in experimental liver regeneration and hepatocarcinogenesis (English shop)

Wolf-Dieter Kuhlmann (Author)
Karlheinz Wurster (Author)


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Table of Contents, PDF (130 KB)

ISBN-13 (Hard Copy) 9783736977754
ISBN-13 (eBook) 9783736967755
Language English
Page Number 92
Lamination of Cover matt
Edition 1.
Publication Place Göttingen
Publication Date 2023-05-25
General Categorization Non-Fiction
Departments Human medicine
Theoretical medicine
Keywords AFP, AFP expression, AFP immuno-expression, AFP purification, AFP recurrence, Alpha-fetoprotein, Animal models, Antibody purification, Antibody-enzyme conjugates, Autoradiography, Bile ductular cells, Biliary epithelial cells, Canal of Hering, Canalicular epithelial cells, Cancer stem cells, Carbon tetrachloride intoxication, Carcinogenesis, Carcinogens, Dedifferentiation, Differentiation, DNA synthesis, Enzyme-linked antibodies, Experimental hepatocarcinogenesis, Galactosamine injury, Hepatic progenitors, Hepatocarcinogenesis, Hepatocyte differentiation, Hepatocyte maturation, Hepatocytes, Histology, Hyperplastic nodules, Immuno-affinity chromatography, Immunocytochemistry, Immunohistology, Immuno-staining, Injury models, Liver cancer, Liver carcinoma, Liver injury, Liver regeneration, Liver repair, Maturation arrest, N-nitrosomorpholine, Oncodevelopment, Oval cells, Partial hepatectomy, Progenitor cells, Proliferation, Retro-differentiation, Stem cells, Stem-like cells, Stem-like progenitors, Stem-like properties, Transdifferentiation, Transitory cell antigens, Tritiated thymidine, AFP, AFP Expression, AFP Immun-Expression, AFP Reinigung, AFP Wiederkehr, Alpha-Fetoprotein, Tiermodelle, Antikörper Aufreinigung, Antikörper-Enzym Konjugate, Autoradiographie, Gallengangzellen, Biliäre Epithelzellen, Kanal von Hering, Hering-Kanal, Kanalikuläre Epithelzellen, Krebsstammzellen, Tetrachlorkohlenstoff, Karzinogenese, Karzinogene, Dedifferenzierung, Differenzierung, DNA Synthese, Enzym-konjugierte Antikörper, Experimentelle Hepatocarcinogenese, Galaktosamin-Schädigung, Hepatische Vorläuferzellen, Hepatocarcinogenese, Hepatozytendifferenzierung, Hepatozytenreifung, Hepatozyten, Histologie, Hyperplastische Knoten, Immunaffinitätschromatographie, Immunzytochemie, Immunhistologie, Immunfärbung, Schädigungsmodelle, Leberkrebs, Leberkarzinom, Leberschädigung, Leber-Regeneration, Leber-Reparatur, Reifungsstopp, Reifungsstillstand, N-Nitrosomorpholin, Onko-fetale Entwicklung, Ovale Zellen, Partielle Hepatektomie, Progenitorzellen, Proliferation, Retro-Differenzierung, Rückdifferenzierung, Stammzellen, Stammähnliche Zellen, Stammähnliche Vorläuferzellen, Stammähnliche Eigenschaften, Transdifferenzierung, Transitorische Zellantigene, Tritiumhaltiges Thymidin, tritiiertes Thymidin

Hepatocytes and bile ductular cells possess stem-like potential in restoring liver tissue. This was derived from rat and mouse injury models such as (a) partial hepatectomy by surgery; (b) acute carbon tetrachloride intoxication; © injuries by D-galactosamine (GalN) or by N-nitrosomorpholine (NNM); (d) experimental hepatocarcinogenesis with NNM using different doses. Liver parenchyma was restored by adult hepatocytes after partial hepatectomy as well as after carbon tetrachloride intoxication. Concurrently, hepatocytes resumed synthesis of alpha-1-fetoprotein (AFP) which depended to a high degree on the animal species and strains used in that studies. In contrast to this way of regeneration, liver repair after heavy injuries by agents such as GalN and NNM was initiated by oval cell proliferation deriving from ductular epithelial cells of the canaliculi of HERING (canals of Hering, intrahepatic bile ductules) as sources of endogenous progenitors. Progenitor populations reach levels of differentiation with fetal gene reactivation and significant AFP expression. Proliferating oval cells operated as transit and amplification compartment during liver regeneration. Oval cell proliferation and concurrent AFP synthesis occurred also in the early stages of hepatocarcinogenesis when the carcinogen NNM was applied in toxic doses. The histologic features compared to those seen in GalN intoxication. NNM carcinogenicity was of concern for both oval cells and adult hepatocytes with risks of transformation and malignant development. At the cancer stage, carcinomas either synthesizing AFP or not can be found side by side in the same liver. AFP expression by cancer cells seems to be a special attribute to differentiation stages in carcinogenesis. Clonality, maturation arrest and retro-differentiation feature cells with high autonomy.