Detektion von Proteinen an funktionalisierten Ultramikroelektroden (English shop)

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The dissertation “Detection of Proteins at Functionalized Ultramicroelectrodes” by Alexander Paul investigates the development and optimization of electrochemical biosensors for sensitive and specific protein detection. The research focuses on the functionalization of gold ultramicroelectrodes, the immobilization of biological recognition molecules, and the amplification of electrochemical detection signals. Different surface modification strategies are evaluated, including self-assembled thiol monolayers, thiolated carboxymethyl cellulose, and dendrimer-based coatings, to reduce nonspecific adsorption and improve antibody immobilization. Particular attention is given to reporter enzymes such as alkaline phosphatase and galactosidases, together with the development of suitable electrochemical substrates. Phage display technology is employed to generate specific antibody fragments against the bacterial food toxins botulinum neurotoxin A and staphylococcal enterotoxin B. Another major focus is the integration of sandwich ELISA methods with functionalized magnetic particles and electrochemical signal amplification through redox recycling. Lysozyme is used as a model protein to optimize the analytical procedure, achieving a detection sensitivity of 10 ng/ml within approximately 50 minutes. Furthermore, staphylococcal enterotoxin B is successfully detected at concentrations of 100 ng/ml. The findings demonstrate that dendrimer-based surface modifications and magnetic particle-assisted immunoassays can improve the analytical performance of miniaturized electrochemical biosensors. The dissertation contributes to the development of sensitive chip-based protein detection technologies with potential applications in food safety analysis, biotechnology, and biochemical diagnostics.