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Leitlinien Unfallchirurgie
5. Auflage bestellen |
Extract, PDF (1.3 MB)
Table of Contents, PDF (620 KB)
ISBN-13 (Hard Copy) | 9783736999138 |
ISBN-13 (eBook) | 9783736989139 |
Language | English |
Page Number | 144 |
Lamination of Cover | matt |
Edition | 1. |
Book Series | Hochschulschriften - Institut für Systembiotechnologie, Universität des Saarlandes |
Volume | 7 |
Publication Place | Göttingen |
Place of Dissertation | Saarbrücken |
Publication Date | 2018-12-06 |
General Categorization | Dissertation |
Departments |
Microbiology and biotechnology
|
Keywords | bio-succinic acid,bacterium Basfia , carbon-three chemicals |
The recent rise of bio-succinic acid as an industrial platform chemical has brought the rumen bacterium Basfia succiniciproducens, a natural producer of this organic acid, into the focus of research. This work investigated the potential of B. succiniciproducens for the production of carbon-three chemicals from sugars. Beside the optimization of improved genetic tools, the C3 chemicals alanine, β-alanine and 3-hydroxypropionate (3-HP) were targeted. A new alanine producer, B. succiniciproducens ALA-1, exhibited good performance in the bioprocess. The next step demonstrated the proof-of-concept for β-alanine synthesis using the heterologous expression of aspartate 1 decarboxylase genes from Corynebacterium glutamicum or Vibrio natriegens. The expression of two additional genes enabled the de novo synthesis of up to 100 mg L-1 3-HP from glucose.