|Book Series (88)||
|Biochemistry, molecular biology, gene technology||113|
|Domestic and nutritional science||44|
|Environmental research, ecology and landscape conservation||147|
5. Auflage bestellen
|ISBN-13 (Hard Copy)||9783736978478|
|Lamination of Cover||glossy|
|Place of Dissertation||Universität Göttingen|
Biochemistry, molecular biology, gene technology
|Keywords||eukaryotic cells, nucleus, compartmentalization, nucleocytoplasmic transport, importin, shuttling, nuclear transport receptors (NTRs), nuclear pore complex (NPC), importins, exportins, biportins, export complex, transport pathways, anti-NTR nanobodies, Transportin 1 (TRN1), Exportin 4 (Xpo4), Exportin 7 (Xpo7), CAS, nanobody fusions, impede nuclear pore passage, transport cycle, inhibitor, inhibition, NTR/cargo complexes, FG phase, NTR-meidated transport, permeabilized cells, Ran binding, transport block, nanobodies (Nbs), nucleoporins (Nups), tag-Nbs, NTR blockers, eukaryotische Zellen, Zellkern, Kompartimentierung, nukleozytoplasmatischer Transport, Kerntransportrezeptoren (NTRs), Kernporenkomplex (NPC), Nanokörperfusionen, NTR-vermeideter Transport, permeabilisierte Zellen, NTR-Blocker|
|URL to External Homepage||https://www.mpinat.mpg.de|
Eukaryotic cells are divided into a nuclear and a cytoplasmic compartment. This separates transcription from translation and makes gene expression dependent on nucleocytoplasmic transport. The members of the importin β superfamily function as shuttling nuclear transport receptors (NTRs) that recognize and actively transport cargoes through nuclear pores. An estimated 5 000 to 10 000 different human proteins are subject to active nuclear transport. Numerous cargo/NTR pairs have been identified, however, we are still far from a complete understanding as it has been very challenging to setup a systematic in vivo analysis that integrates the impact of all transport pathways.
In this study, we obtained anti-NTR nanobodies against TRN1, Xpo4, Xpo7, and CAS. Our aim was to identify nanobodies, and prepare nanobody fusions, that impede nuclear pore-passage of the targeted NTR and thus, interrupt a given transport cycle. These nanobody fusions were observed to inhibit the partition of NTR/cargo complexes into a reconstituted FG phase. We also observed that the nanobodies and nanobody fusions inhibit NTR transport in permeabilized cells.