Numerische Untersuchungen zu mechanischen Beanspruchungen von Chinese-Hamster-Ovary Zellen in Mikrokanälen (Volume 2) (English shop)

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Chinese Hamster Ovary (CHO) cells are used to produce active pharmaceutical ingredients in the pharmaceutical industry and play an important role in basic research. Within the SysCompart project funded by the German Federal Ministry of Education and Research, a microfluidic lab-on-a-chip was developed in which such CHO cells are mechanically disrupted. As part of a suspension conveyed through microchannels, these cells encounter spatial barriers. In this work, the stress acting on these cells at the barrier front due to the surrounding flow was analysed and characterised by means of numerical flow simulations. In addition, flow conditions for detaching adhering CHO cells and thus also friction coefficients could be determined. Furthermore, modelling approaches and simulation results on the deformation behaviour of these cells are presented, based on established models of continuum theory and the discrete element method.